| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Thalidomide protects mice against LPS-induced shock
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| Autores: |
Moreira,A.L.
Wang,J.
Sarno,E.N.
Kaplan,G.
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| Data: |
1997-10-01
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| Ano: |
1997
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| Palavras-chave: |
Endotoxic shock
Thalidomide
Cytokines
Inflammation
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| Resumo: |
Thalidomide has been shown to selectively inhibit TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font> production in vitro by lipopolysaccharide (LPS)-stimulated monocytes. TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font> has been shown to play a pivotal role in the pathophysiology of endotoxic shock. Using a mouse model of LPS-induced shock, we investigated the effects of thalidomide on the production of TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font> and other cytokines and on animal survival. After injection of 100-350 µg LPS into mice, cytokines including TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font>, IL-6, IL-10, IL-1ß, GM-CSF and IFN-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">g</font> were measured in the serum. Administration of 200 mg/kg thalidomide to mice before LPS challenge modified the profile of LPS-induced cytokine secretion. Serum TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font> levels were reduced by 93%, in a dose-dependent manner, and TNF-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font> mRNA expression in the spleens of mice was reduced by 70%. Serum IL-6 levels were also inhibited by 50%. Thalidomide induced a two-fold increase in serum IL-10 levels. Thalidomide treatment did not interfere with the production of GM-CSF, IL-1ß or IFN-<!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">g</font>. The LD50 of LPS in this model was increased by thalidomide pre-treatment from 150 µg to 300 µg in 72 h. Thus, at otherwise lethal doses of LPS, thalidomide treatment was found to protect animals from death
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000010
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X1997001000010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.30 n.10 1997
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| Direitos: |
info:eu-repo/semantics/openAccess
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